概述

  • 产品名称Anti-EDF1抗体
    参阅全部 EDF1 一抗
  • 描述
    兔多克隆抗体to EDF1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (AESDWDTVTV LRKKGPTAAQ AKSKQAILAA QRRGEDVETS KKWAAGQNKQ) of Human EDF1 (NP_003783).

  • 阳性对照
    • THP1 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab95110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
  • 组织特异性Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
  • 序列相似性Contains 1 HTH cro/C1-type DNA-binding domain.
  • 发展阶段Expressed in fetal tissues. More abundant in kidney.
  • 结构域The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein.
  • 翻译后修饰Phosphorylated (by PKA and PKC).
  • 细胞定位Cytoplasm. Nucleus. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP19 antibody
    • EDF-1 antibody
    • edf1 antibody
    • EDF1_HUMAN antibody
    • Endothelial differentiation related factor 1 antibody
    • Endothelial differentiation-related factor 1 antibody
    • MBF1 antibody
    • MGC9058 antibody
    • Multiprotein bridging factor 1 antibody
    • Multiprotein-bridging factor 1 antibody
    see all

Anti-EDF1 antibody 图像

  • Immunofluorescence analysis of human fibroblasts fixed with methanol labelling EDF1 with ab95110 at 5ug/ml.

  • Anti-EDF1 antibody (ab95110) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa
    Gel concentration: 10-20%

Anti-EDF1 antibody (ab95110)参考文献

ab95110 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95110.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"