概述

  • 产品名称
  • 描述
    兔多克隆抗体to EDF1
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (AESDWDTVTV LRKKGPTAAQ AKSKQAILAA QRRGEDVETS KKWAAGQNKQ) of Human EDF1 (NP_003783).

  • 阳性对照
    • THP1 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab95110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
  • 组织特异性
    Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
  • 序列相似性
    Contains 1 HTH cro/C1-type DNA-binding domain.
  • 发展阶段
    Expressed in fetal tissues. More abundant in kidney.
  • 结构域
    The IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein.
  • 翻译后修饰
    Phosphorylated (by PKA and PKC).
  • 细胞定位
    Cytoplasm. Nucleus. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAP19 antibody
    • EDF-1 antibody
    • edf1 antibody
    • EDF1_HUMAN antibody
    • Endothelial differentiation related factor 1 antibody
    • Endothelial differentiation-related factor 1 antibody
    • MBF1 antibody
    • MGC9058 antibody
    • Multiprotein bridging factor 1 antibody
    • Multiprotein-bridging factor 1 antibody
    see all

Anti-EDF1 antibody 图像

  • Immunofluorescence analysis of human fibroblasts fixed with methanol labelling EDF1 with ab95110 at 5ug/ml.

  • Anti-EDF1 antibody (ab95110) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa
    Gel concentration: 10-20%

Anti-EDF1 antibody (ab95110)参考文献

ab95110 has not yet been referenced specifically in any publications.

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