概述

  • 产品名称
    Anti-EDD抗体
    参阅全部 EDD 一抗
  • 描述
    山羊多克隆抗体to EDD
  • 宿主
    Goat
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide: LAIKTKNFGFV, corresponding to C terminal amino acids 2789-2799 of Human EDD.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明
    GenBank Accession Number – NP_056986. LocusLink ID - 51366 (human); 70790 (mouse); 117060 (rat). Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; cell proliferation; protein modification; soluble fraction; nucleus; ligase activity.

性能

应用

Our Abpromise guarantee covers the use of ab4376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).Can be blocked with Human EDD peptide (ab23029).
IP Use at an assay dependent dilution.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. No results could not be obtained after antigen retrieval at pH6.
ICC/IF Use at an assay dependent concentration. PubMed: 21148289

靶标

  • 功能
    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
  • 组织特异性
    Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all

图片

  • Western blot - Anti-EDD antibody (ab4376)
    Western blot - Anti-EDD antibody (ab4376)This image is courtesy of an anonymous Abreview
    Anti-EDD antibody (ab4376) at 1/500 dilution + MDA-MB-468 breast cancer cells (25µg)

    Secondary
    Donkey anti-goat conjugated to HRP at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size: 309 kDa


    Exposure time: 5 seconds


    Developed using SuperBlock Blocking Buffer (pierce) and pico ECL kit (pierce) to reduce the backgound observed - please refer to abreview for further experimental details.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab4376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab4376)
    ab4376 (4µg/ml) staining of paraffin embedded Human Testis shows nuclear staining . Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

文献

This product has been referenced in:
  • Hay-Koren A  et al. The EDD E3 ubiquitin ligase ubiquitinates and up-regulates beta-catenin. Mol Biol Cell 22:399-411 (2011). WB ; Human . Read more (PubMed: 21118991) »
  • Won JH  et al. Phenotypic changes in mouse pancreatic stellate cell Ca2+ signaling events following activation in culture and in a disease model of pancreatitis. Mol Biol Cell 22:421-36 (2011). ICC/IF . Read more (PubMed: 21148289) »

See all 2 Publications for this product

发表研究结果有使用 ab4376？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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11 reviews or Q&A

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Votes: Highest First

Western blot abreview for Anti-EDD antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
50 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (7% Tris-Bicine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
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提交于 Sep 28 2009

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (lung tissue)
Total protein in input
1000 µg
Specification
lung tissue
Immuno-precipitation step
Protein A/G
Read More
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Abcam user community

Verified customer

提交于 Oct 31 2008

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rabbit Tissue lysate - whole (heart tissue)
Total protein in input
1000 µg
Specification
heart tissue
Immuno-precipitation step
Protein A/G
Read More
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Abcam user community

Verified customer

提交于 Oct 31 2008

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (lung tissue)
Total protein in input
1000 µg
Specification
lung tissue
Immuno-precipitation step
Protein A/G
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Oct 20 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
50 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
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Abcam user community

Verified customer

提交于 Aug 12 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount
30 µg
Specification
NIH 3T3
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
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提交于 Jun 12 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 cell line)
Loading amount
25 µg
Specification
C6 cell line
Gel Running Conditions
Reduced Denaturing (4% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
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提交于 Jun 04 2008

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (breast cancer cells)
Total protein in input
250 µg
Specification
breast cancer cells
Immuno-precipitation step
Protein A/G
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 May 23 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (T98G cell line)
Loading amount
25 µg
Specification
T98G cell line
Gel Running Conditions
Reduced Denaturing (6% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 May 02 2008

Western blot abreview for Anti-EDD antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-468 breast cancer cells)
Loading amount
25 µg
Specification
MDA-MB-468 breast cancer cells
Gel Running Conditions
Reduced Denaturing
Blocking step
SuperBlock Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Mar 12 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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