Customer reviews and FAQs

概述

  • 产品名称Anti-EDD抗体
    参阅全部 EDD 一抗
  • 描述
    山羊多克隆抗体to EDD
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide: LAIKTKNFGFV, corresponding to C terminal amino acids 2789-2799 of Human EDD.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明GenBank Accession Number – NP_056986. LocusLink ID - 51366 (human); 70790 (mouse); 117060 (rat). Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; cell proliferation; protein modification; soluble fraction; nucleus; ligase activity.

性能

应用

Our Abpromise guarantee covers the use of ab4376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).Can be blocked with Human EDD peptide (ab23029).
IP Use at an assay dependent dilution.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. No results could not be obtained after antigen retrieval at pH6.
ICC/IF Use at an assay dependent concentration. PubMed: 21148289

靶标

  • 功能E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
  • 组织特异性Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all

Anti-EDD antibody 图像

  • Western blot - EDD antibody (ab4376)
    Western blot - EDD antibody (ab4376)
    Anti-EDD antibody (ab4376) at 1/500 dilution + MDA-MB-468 breast cancer cells (25µg)

    Secondary
    Donkey anti-goat conjugated to HRP at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size : 309 kDa


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab4376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab4376)
    ab4376 (4µg/ml) staining of paraffin embedded Human Testis shows nuclear staining . Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

Anti-EDD antibody (ab4376)参考文献

This product has been referenced in:
  • Hay-Koren A  et al. The EDD E3 ubiquitin ligase ubiquitinates and up-regulates beta-catenin. Mol Biol Cell 22:399-411 (2011). WB ; Human . Read more (PubMed: 21118991) »
  • Won JH  et al. Phenotypic changes in mouse pancreatic stellate cell Ca2+ signaling events following activation in culture and in a disease model of pancreatitis. Mol Biol Cell 22:421-36 (2011). ICC/IF . Read more (PubMed: 21148289) »

See all 2 Publications for this product

发表研究结果有使用 ab4376？请让我们知道，以便我们可以引用本数据表中的参考文章。

Product Wall

提交 ab4376 的 abreview 提交 ab4376 的问题

11 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-EDD antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (7% Tris-Bicine Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Sep 28 2009

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (lung tissue)
Total protein in input 1000 µg
Specification lung tissue
Immuno-precipitation step Protein A/G
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Oct 31 2008

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rabbit Tissue lysate - whole (heart tissue)
Total protein in input 1000 µg
Specification heart tissue
Immuno-precipitation step Protein A/G
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Oct 31 2008

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (lung tissue)
Total protein in input 1000 µg
Specification lung tissue
Immuno-precipitation step Protein A/G
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Oct 20 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 50 µg
Specification heart
Gel Running Conditions Reduced Denaturing (6% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Aug 12 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount 30 µg
Specification NIH 3T3
Gel Running Conditions Reduced Denaturing (6% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Jun 12 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (C6 cell line)
Loading amount 25 µg
Specification C6 cell line
Gel Running Conditions Reduced Denaturing (4% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Jun 04 2008

Immunoprecipitation abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (breast cancer cells)
Total protein in input 250 µg
Specification breast cancer cells
Immuno-precipitation step Protein A/G
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 May 23 2008

Western blot abreview for Anti-EDD antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (T98G cell line)
Loading amount 25 µg
Specification T98G cell line
Gel Running Conditions Reduced Denaturing (6% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 May 02 2008

Western blot abreview for Anti-EDD antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB-468 breast cancer cells)
Loading amount 25 µg
Specification MDA-MB-468 breast cancer cells
Gel Running Conditions Reduced Denaturing
Blocking step SuperBlock Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Mar 12 2008

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"