概述

  • 产品名称Anti-EDC3抗体
    参阅全部 EDC3 一抗
  • 描述
    小鼠单克隆抗体to EDC3
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-509 of Human EDC3

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Binds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis.
  • 组织特异性Expressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland.
  • 序列相似性Belongs to the EDC3 family.
    Contains 1 DFDF domain.
    Contains 1 YjeF N-terminal domain.
  • 结构域The DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6.
  • 细胞定位Cytoplasm > P-body. Processing bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EDC 3 antibody
    • edc3 antibody
    • EDC3_HUMAN antibody
    • enhancer of mRNA decapping 3 homolog (S. cerevisiae) antibody
    • Enhancer of mRNA decapping 3 homolog antibody
    • Enhancer of mRNA decapping 3, S. cerevisiae, homolog of antibody
    • Enhancer of mRNA decapping protein 3 antibody
    • Enhancer of mRNA-decapping protein 3 antibody
    • FLJ21128 antibody
    • FLJ31777 antibody
    • hYjeF_N2 antibody
    • hYjeF_N2-15q23 antibody
    • LSM 16 antibody
    • LSM16 antibody
    • LSM16 homolog (EDC3 S. cerevisiae) antibody
    • LSM16 homolog (EDC3, S. cerevisiae) antibody
    • LSM16 homolog antibody
    • YJDC antibody
    • YjeF domain containing antibody
    • YjeF domain containing protein 1 antibody
    • YjeF domain containing protein antibody
    • YjeF domain-containing protein 1 antibody
    • YjeF N-terminal domain-containing protein 2 antibody
    • YjeF_N2 antibody
    • YJEFN2 antibody
    see all

Anti-EDC3 antibody 图像

  • Overlay histogram showing HeLa cells stained with ab57780 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57780, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • EDC3 antibody (ab57780) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-EDC3 antibody (ab57780)参考文献

This product has been referenced in:

See all 4 Publications for this product

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