The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能Binds single-stranded RNA. In the process of mRNA degradation, may play a role in mRNA decapping. May play a role in spermiogenesis and oogenesis.
组织特异性Expressed in theca and granulosa cells in ovary, and in spermatids of the meiotic division part II and apical membrane of Sertoli cells in testis (at protein level). Also expressed in brain and mammary gland.
序列相似性Belongs to the EDC3 family. Contains 1 DFDF domain. Contains 1 YjeF N-terminal domain.
结构域The DFDF domain is unstructured by itself. It assumes a helical fold upon interaction with DDX6.
enhancer of mRNA decapping 3 homolog (S. cerevisiae) antibody
Enhancer of mRNA decapping 3 homolog antibody
Enhancer of mRNA decapping 3, S. cerevisiae, homolog of antibody
Enhancer of mRNA decapping protein 3 antibody
Enhancer of mRNA-decapping protein 3 antibody
LSM 16 antibody
LSM16 homolog (EDC3 S. cerevisiae) antibody
LSM16 homolog (EDC3, S. cerevisiae) antibody
LSM16 homolog antibody
YjeF domain containing antibody
YjeF domain containing protein 1 antibody
YjeF domain containing protein antibody
YjeF domain-containing protein 1 antibody
YjeF N-terminal domain-containing protein 2 antibody
Anti-EDC3 antibody 图像
Flow Cytometry - Anti-EDC3 antibody (ab57780)
Overlay histogram showing HeLa cells stained with ab57780 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57780, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - EDC3 antibody (ab57780)
EDC3 antibody (ab57780) at 1ug/lane + HeLa cell lysate at 25ug/lane.