antigen sequence: GQKAQSIGIF SDGDSREINL LQGYKIGVKN LLRPEVRDFW EKLGSYVATE EEGGHVDFFV PLGASEAGIE VLSQ, corresponding to amino acids 335-408 of Human ECT2L.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May act as a guanine nucleotide exchange factor (GEF).
Contains 1 DH (DBL-homology) domain.
Contains 1 F-box domain.
Information by UniProt
- ARHGEF32 antibody
- C6orf91 antibody
- dJ509I19.2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ECT2L antibody (ab122281)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122281 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECT2L antibody (ab122281)
ab122281, at 1/300 dilution, staining ECT2L in paraffin-embedded Human rectum tissue by
has not yet been referenced specifically in any publications.
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