Anti-ECT2 (phospho T790)抗体(ab51494)

概述

  • 产品名称Anti-ECT2 (phospho T790)抗体
    参阅全部 ECT2 一抗
  • 描述
    兔多克隆抗体to ECT2 (phospho T790)
  • 特异性Reactivity occurs against human ECT2 protein at the pT790 residue.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Dog, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide:

    TKDMDSTLSRA

    , corresponding to amino acids 785-795 of Human ECT2

  • 阳性对照
    • HeLa cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab51494 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC/IF
  • 应用说明ELISA: 1/10000 - 1/40000.
    ICC/IF: Use at an assay dependent dilution.
    WB: 1/1000 - 1/4000. Predicted molecular weight: 100 kDa.
    Less than 2% reactivity is observed against the non-phosphorylated form of
    the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
    • 细胞定位ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis
    • 数据库链接
    • 别名
      • ECT 2 antibody
      • ECT2 protein antibody
      • Epithelial cell transforming sequence 2 antibody
      • Epithelial cell transforming sequence 2 oncogene antibody
      • Epithelial cell transforming sequence 2 oncogene protein antibody
      • FLJ10461 antibody
      • MGC138291 antibody
      see all

    Anti-ECT2 (phospho T790) antibody (ab51494)参考文献

    ab51494 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa cells)
    Specification HeLa cells
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 29 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"