概述

  • 产品名称Anti-ECSIT抗体
    参阅全部 ECSIT 一抗
  • 描述
    兔多克隆抗体to ECSIT
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Human
  • 免疫原

    Synthetic peptide: TGMEDPTQPH IVGIQSPDQQ AALARHNPSR PVFVEGPFPL WLRNKCVYYH , corresponding to amino acids 254-303 of Human ECSIT

  • 阳性对照
    • NIH 3T3 lysate.

性能

应用

Our Abpromise guarantee covers the use of ab66380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using a peptide based assay was 1/1562500.

靶标

  • 功能Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
  • 序列相似性Belongs to the ECSIT family.
  • 细胞定位Cytoplasm. Nucleus. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ecsit antibody
    • ECSIT homolog antibody
    • ECSIT protein antibody
    • ECSIT_HUMAN antibody
    • Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial antibody
    • Evolutionarily conserved signaling intermediate in Toll pathways antibody
    • Likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved antibody
    • Protein SITPEC antibody
    • Signaling intermediate in Toll pathway evolutionarily conserved antibody
    • SITPEC antibody
    • SITPEC protein antibody
    see all

Anti-ECSIT antibody 图像

  • Anti-ECSIT antibody (ab66380) at 1.25 µg/ml + NIH 3T3 lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Anti-ECSIT antibody (ab66380)参考文献

ab66380 has not yet been referenced specifically in any publications.

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