Anti-ECSIT抗体(ab21288)
Key features and details
- Rabbit polyclonal to ECSIT
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ECSIT抗体 -
描述
兔多克隆抗体to ECSIT -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to 14 amino acids near the C-terminus of ECSIT (Human).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration. PubMed: 21163940
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ICC/IF |
Use at an assay dependent concentration. PubMed: 21163940
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 49 kDa).
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说明 |
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IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. PubMed: 21163940 |
ICC/IF
Use at an assay dependent concentration. PubMed: 21163940 |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 49 kDa). |
靶标
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功能
Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development (By similarity). Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. -
序列相似性
Belongs to the ECSIT family. -
细胞定位
Cytoplasm. Nucleus. Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 51295 Human
- Entrez Gene: 26940 Mouse
- Omim: 608388 Human
- SwissProt: Q9BQ95 Human
- SwissProt: Q9QZH6 Mouse
- Unigene: 515146 Human
- Unigene: 425568 Mouse
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别名
- Ecsit antibody
- ECSIT homolog antibody
- ECSIT protein antibody
see all
图片
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Lane 1 : Anti-ECSIT antibody (ab21288) at 0.5 µg/ml
Lane 2 : Anti-ECSIT antibody (ab21288) at 1 µg/ml
Lane 3 : Anti-ECSIT antibody (ab21288) at 2 µg/ml
All lanes : human heart lysate
Predicted band size: 49 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab21288 被引用在 7 文献中.
- Marrocco A et al. Metabolic Adaptation of Macrophages as Mechanism of Defense against Crystalline Silica. J Immunol 207:1627-1640 (2021). PubMed: 34433619
- Meng H et al. Synthetic VSMCs induce BBB disruption mediated by MYPT1 in ischemic stroke. iScience 24:103047 (2021). PubMed: 34553133
- Seo JH et al. MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection. Sci Rep 10:11183 (2020). PubMed: 32636430
- Kim MJ et al. CRBN Is a Negative Regulator of Bactericidal Activity and Autophagy Activation Through Inhibiting the Ubiquitination of ECSIT and BECN1. Front Immunol 10:2203 (2019). PubMed: 31620128
- Guarani V et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). PubMed: 24344204
- Soler-López M et al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, ICC/IF, IP ; Human . PubMed: 21163940
- Vogel RO et al. Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly. Genes Dev 21:615-24 (2007). PubMed: 17344420