概述

  • 产品名称Anti-Eco1抗体
    参阅全部 Eco1 一抗
  • 描述
    小鼠多克隆抗体to Eco1
  • 特异性This antibody is reactive against mammalian transfected lysate.
  • 经测试应用适用于: WBmore details
  • 免疫原

    Recombinant fragment corresponding to Human Eco1 aa 669-840 (C terminal).
    Sequence:

    MVLPEDPKYALKKVDEIREMVDNDLGFQQAPLMCYSRTKTLLFISNDKKV VGCLIAEHIQWGYRVIEEKLPVIRSEEEKVRFERQKAWCCSTLPEPAICG ISRIWVFSMMRRKKIASRMIECLRSNFIYGSYLSKEEIAFSDPTPDGKLF ATQYCGTGQFLVYNFINGQNST


    Database link: Q5FWF5

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab70442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 95 kDa).

The actual band size corresponds to the size of the immunizing protein fragment which was transfected into lysate. We do not yet have data to indicate that the antibody will work in endogenously expressed samples.

靶标

  • 功能Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3.
  • 组织特异性Widely expressed. Expressed in heart, brain, liver, placenta, lung, kidney and pancreas. Highly expressed in muscle.
  • 序列相似性Belongs to the acetyltransferase family. GCN5 subfamily.
  • 结构域The N-terminal region seems to be responsible for the association with chromosomes, thus excluding any involvement of the Zn finger in this process.
  • 翻译后修饰Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Chromosome. Nuclear at interphase, associated with chromosomes during mitosis.
  • Information by UniProt
  • 别名
    • A930014I12Rik antibody
    • CTF antibody
    • CTF7 homolog 1 antibody
    • CTF7, S. cerevisiae, homolog of, 1 antibody
    • ECO1 antibody
    • ECO1 homolog 1 antibody
    • ECO1, S. cerevisiae, homolog of, 1 antibody
    • EFO1 antibody
    • EFO1p antibody
    • ESCO1 antibody
    • ESCO1_HUMAN antibody
    • ESO1 antibody
    • ESO1 homolog 1 antibody
    • ESO1, S. pombe, homolog of, 1 antibody
    • Establishment factor like protein 1 antibody
    • Establishment factor ortholog 1 antibody
    • Establishment factor-like protein 1 antibody
    • Establishment of cohesion 1 homolog 1 antibody
    • Establishment of cohesion 1, S. cerevisiae, homolog of antibody
    • establishment of sister chromatid cohesion N-acetyltransferase 1 antibody
    • hEFO1 antibody
    • HGNC:24645 antibody
    • KIAA1911 antibody
    • N acetyltransferase ESCO1 antibody
    • N-acetyltransferase ESCO1 antibody
    see all

Anti-Eco1 antibody 图像

  • All lanes : Anti-Eco1 antibody (ab70442) at 1/500 dilution

    Lane 1 : Eco1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 95 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)

    Actual band size corresponds to the size of the transfected immunizing protein fragment.

Anti-Eco1 antibody (ab70442)参考文献

ab70442 has not yet been referenced specifically in any publications.

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