The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 95 kDa).
The actual band size corresponds to the size of the immunizing protein fragment which was transfected into lysate. We do not yet have data to indicate that the antibody will work in endogenously expressed samples.
功能Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3.
组织特异性Widely expressed. Expressed in heart, brain, liver, placenta, lung, kidney and pancreas. Highly expressed in muscle.
序列相似性Belongs to the acetyltransferase family. GCN5 subfamily.
结构域The N-terminal region seems to be responsible for the association with chromosomes, thus excluding any involvement of the Zn finger in this process.
翻译后修饰Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus. Chromosome. Nuclear at interphase, associated with chromosomes during mitosis.