重组Anti-ECHS1抗体[EPR11785] (ab174312)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11785] to ECHS1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ECHS1抗体[EPR11785]
参阅全部 ECHS1 一抗 -
描述
兔单克隆抗体[EPR11785] to ECHS1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- MCF7, HeLa, 293T, HepG2, Human fetal liver and fetal muscle lysates; Human heart tissue; Human liver tissue; MCF7 cell
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11785 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174312于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 31 kDa.
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ICC/IF |
1/250 - 1/500.
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说明 |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 31 kDa. |
ICC/IF
1/250 - 1/500. |
靶标
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相关性
ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway. -
细胞定位
Mitochondrion matrix. -
数据库链接
- Entrez Gene: 1892 Human
- Omim: 602292 Human
- SwissProt: P30084 Human
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别名
- C80529 antibody
- EC 4.2.1.17 antibody
- ECHS 1 antibody
see all
图片
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All lanes : Anti-ECHS1 antibody [EPR11785] (ab174312) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Human fetal liver tissue lysate
Lane 6 : Human fetal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa -
Immunofluorescent staining of MCF7 cells labeling ECHS1 with ab174312 at 1/250 dilution (red). DAPI nuclear staining (blue).
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Immunohistochemical analysis of paraffin embedded Human liver tissue labaling ECHS1 with ab174312 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ECHS1 with ab174312 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab174312 被引用在 2 文献中.
- Simon MT et al. ECHS1 disease in two unrelated families of Samoan descent: Common variant - rare disorder. Am J Med Genet A 185:157-167 (2021). PubMed: 33112498
- Mikalayeva V et al. Fatty Acid Synthesis and Degradation Interplay to Regulate the Oxidative Stress in Cancer Cells. Int J Mol Sci 20:N/A (2019). PubMed: 30889783