Anti-ECHS1抗体(ab153732)
Key features and details
- Rabbit polyclonal to ECHS1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ECHS1抗体
参阅全部 ECHS1 一抗 -
描述
兔多克隆抗体to ECHS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 55-290 of Human ECHS1 (Uniprot ID: P30084).
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阳性对照
- NT2D1, PC3, U-87 MG, SK N SH, Neuro 2A, GL261 and C8D30 whole cell lysates; Human NCI-N87 xenograft tissue; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab153732于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer |
ICC/IF
1/100 - 1/1000. |
靶标
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相关性
ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway. -
细胞定位
Mitochondrion matrix. -
数据库链接
- Entrez Gene: 1892 Human
- Entrez Gene: 93747 Mouse
- Omim: 602292 Human
- SwissProt: P30084 Human
- SwissProt: Q8BH95 Mouse
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别名
- C80529 antibody
- EC 4.2.1.17 antibody
- ECHS 1 antibody
see all
图片
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Immunofluorescent analysis of methanol-fixed HeLa cells, labeling ECHS1 with ab153732 at 1/200 dilution. Right cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue, labeling ECHS1 with ab153732 at 1/250 dilution.
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All lanes : Anti-ECHS1 antibody (ab153732) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa
12% SDS PAGE -
All lanes : Anti-ECHS1 antibody (ab153732) at 1/3000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : PC3 whole cell lysate
Lane 3 : U-87 MG whole cell lysate
Lane 4 : SK N SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa
12% SDS PAGE
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab153732 被引用在 1 文献中.
- Yamada K et al. Clinical, biochemical and metabolic characterisation of a mild form of human short-chain enoyl-CoA hydratase deficiency: significance of increased N-acetyl-S-(2-carboxypropyl)cysteine excretion. J Med Genet 52:691-8 (2015). PubMed: 26251176