概述

  • 产品名称Anti-ECHDC2抗体
    参阅全部 ECHDC2 一抗
  • 描述
    兔多克隆抗体to ECHDC2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A synthetic peptide within the range of amino acids 122-152 of human ECHDC2

  • 阳性对照
    • 721_B cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.Can be blocked with ECHDC2 peptide (ab195585). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 相关性ECHDC2 belongs to the enoyl-CoA hydratase/isomerase family. There are two named isforms.
  • 细胞定位Mitochondrion
  • 数据库链接
  • 别名
    • 1300017C12Rik antibody
    • 2610009M20Rik antibody
    • D4Ertd765e antibody
    • Enoyl Coenzyme A hydratase domain containing 2 antibody
    • FLJ10948 antibody
    • RGD1308525 antibody
    • RP23-379K6.3 antibody
    see all

Anti-ECHDC2 antibody 图像

  • Anti-ECHDC2 antibody (ab75788) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-ECHDC2 antibody (ab75788)参考文献

This product has been referenced in:
  • Du J  et al. Enoyl coenzyme a hydratase domain-containing 2, a potential novel regulator of myocardial ischemia injury. J Am Heart Assoc 2:e000233 (2013). Rat . Read more (PubMed: 24108764) »

See 1 Publication for this product

Product Wall

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (heart, liver, lung brain)
Loading amount 30 µg
Specification heart, liver, lung brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Yang Shi

Verified customer

提交于 Sep 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"