Anti-ECH1抗体[9D11AF3] (ab110294)

概述

  • 产品名称Anti-ECH1抗体[9D11AF3]
    参阅全部 ECH1 一抗
  • 描述
    小鼠单克隆抗体[9D11AF3] to ECH1
  • 经测试应用适用于: ICC/IF, In-Cell ELISA, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    human liver mitochondria

  • 阳性对照
    • HDFn cells and HL-60 cells
  • 常规说明

    ECH1 is a member of the hydratase/isomerase superfamily involved in fatty acid metabolism. It is localized to peroxisomes and/or mitochondria in different mammals. In humans, levels of ECH1 are modulated by peroxisome proliferators. As the name implies, ECH1 catalyzes the isomerization of cis ?3,5, double bonds in unsaturated acyl-CoAs to yield trans ?2,4-dienoyl CoAs. These are substrates for ?2,4-dienoyl CoA reductase which produces trans ?3 enoyl CoA. This intermediate can then be further transformed by ?3, ?2 - enoyl CoA isomerase to finally yield the trans-2 double bond configured fatty acyl CoA, which can be then directed into the ß-oxidation pathway. Expression of ECH1 is highest in metabolically active tissues such as heart, skeletal muscle, central nervous system, liver and kidney. Recent studies show that it is down regulated in gastric carcinoma and CLL.

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

靶标

  • 功能Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
  • 通路Lipid metabolism; fatty acid beta-oxidation.
  • 序列相似性Belongs to the enoyl-CoA hydratase/isomerase family.
  • 细胞定位Mitochondrion. Peroxisome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4)-dienoyl-CoA isomerase antibody
    • 5)-Delta(2 antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
    • Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial antibody
    • Delta(3 antibody
    • Delta3 5 delta2 4 dienoyl CoA isomerase antibody
    • Dienoyl CoA isomerase antibody
    • ECH 1 antibody
    • Ech1 antibody
    • ECH1_HUMAN antibody
    • Enoyl Coenzyme A hydratase 1 antibody
    • Enoyl Coenzyme A hydratase 1 peroxisomal antibody
    • HPXEL antibody
    • mitochondrial antibody
    • MS1034 antibody
    • Peroxisomal enoyl CoA hydratase 1 antibody
    • Peroxisomal enoyl coenzyme A hydratase like protein antibody
    see all

Anti-ECH1 antibody [9D11AF3] 图像

  • HL-60 cells were stained with 1 µg/mL ab110294 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry image of ab110294 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (9D11AF3, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the mitochondria.

Anti-ECH1 antibody [9D11AF3] (ab110294)参考文献

ab110294 has not yet been referenced specifically in any publications.

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