Anti-ECE1抗体(ab71829)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ECE1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Guinea pig, Cow, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human ECE1.

    (Peptide available as ab87264.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HUVEC MEF1 NIH 3T3

性能

应用

Our Abpromise guarantee covers the use of ab71829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 87 kDa).

靶标

  • 相关性
    Function: Converts big endothelin-1 to endothelin-1. Tissue specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Disease: Hirschsprung disease cardiac defects and autonomic dysfunction Similarity: Belongs to the peptidase M13 family.
  • 细胞定位
    Cell Membrane. Single pass type II membrane protein.
  • 数据库链接
  • 别名
    • ECE 1 antibody
    • ECE antibody
    • ECE-1 antibody
    • ECE1 antibody
    • ECE1_HUMAN antibody
    • Endothelin converting enzyme 1 antibody
    • Endothelin-converting enzyme 1 antibody
    see all

图片

  • All lanes : Anti-ECE1 antibody (ab71829) at 1 µg/ml

    Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 87 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab71829 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (DLD-1 colon cancer cells)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
50 µg
Treatment
See comments
Specification
DLD-1 colon cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization
Yes - Tween-20
Specification
pancreas tumor
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
Yes - Tween-20
Specification
colon
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 29 2015


I am very pleased to hear you would like to accept our offer and test ab71829 in monkey]. This code will give you: 1 free PRIMARY ANTIBODY OR $337.00 OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for monk...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册