使用敲除细胞株进行验证RabMAb

Anti-EBP50抗体[EPR5562] (ab109430)

概述

  • 产品名称Anti-EBP50抗体[EPR5562]
    参阅全部 EBP50 一抗
  • 描述
    兔单克隆抗体[EPR5562] to EBP50
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • HepG2, 293T, Jurkat, C6, PC-12 and MCF-7 cell lysates; Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
    • 组织特异性Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
    • 疾病相关Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
    • 序列相似性Contains 2 PDZ (DHR) domains.
    • 翻译后修饰Phosphorylated on serine residues.
    • 细胞定位Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EBP 50 antibody
      • EBP50 antibody
      • Ezrin radixin moesin binding phosphoprotein 50 antibody
      • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
      • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
      • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
      • Na+/H+ exchange regulatory co factor antibody
      • NHERF 1 antibody
      • NHERF antibody
      • NHERF-1 antibody
      • NHERF1 antibody
      • NHRF1_HUMAN antibody
      • NPHLOP2 antibody
      • Regulatory cofactor of Na(+)/H(+) exchanger antibody
      • Slc9a3r1 antibody
      • Sodium hydrogen exchanger regulatory factor 1 antibody
      • Sodium-hydrogen exchanger regulatory factor 1 antibody
      • Sodium/hydrogen exchanger regulatory factor 1 antibody
      • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
      • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
      • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
      • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
      • Solute carrier family 9 member 3 regulator 1 antibody
      see all

    Anti-EBP50 antibody [EPR5562] 图像



    • Predicted band size : 39 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: EBP50 knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109430 observed at 48 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab109430 was shown to recognize EBP50 when EBP50 knockout samples were used, along with additional cross-reactive bands. Wild-type and EBP50 knockout samples were subjected to SDS-PAGE. ab109430 and ab18058 (loading control to Vinculin) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemical analysis of EBP50 in paraffin-embedded Human kidney tissue using ab109430 at 1/100 dilution.
    • All lanes : Anti-EBP50 antibody [EPR5562] (ab109430) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : PC12 cell lysate
      Lane 6 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa

    Anti-EBP50 antibody [EPR5562] (ab109430)参考文献

    ab109430 has not yet been referenced specifically in any publications.

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