概述

  • 产品名称Anti-EBP50抗体
    参阅全部 EBP50 一抗
  • 描述
    兔多克隆抗体to EBP50
  • 特异性Detects EBP 50 from human T84 and Calu3 cells as well as recombinant human protein.
  • 经测试应用适用于: ICC/IF, IHC-Fr, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
    预测可用于: Rabbit, Cow, Cynomolgus Monkey
  • 免疫原

    Recombinant fragment (His-tag) corresponding to Human EBP50. All His-tag specific antibody has been removed.
    Sequence:

    RSASSDTSEELNSQDSP

  • 阳性对照
    • T84 cell extract, mouse airway epithelium

性能

应用

Our Abpromise guarantee covers the use of ab3452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 15703401
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
  • 组织特异性Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
  • 疾病相关Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
  • 序列相似性Contains 2 PDZ (DHR) domains.
  • 翻译后修饰Phosphorylated on serine residues.
  • 细胞定位Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

Anti-EBP50 antibody 图像

  • All lanes : Anti-EBP50 antibody (ab3452) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from human HepG2 cells
    Lane 2 : Whole cell lysate prepared from human HepG2 cells

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 40 seconds

    This image is courtesy of an anonymous abreview.

    See Abreview

  • All lanes : Anti-EBP50 antibody (ab3452) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from mouse liver
    Lane 2 : Whole tissue lysate prepared from mouse liver

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous abreview.

    See Abreview



  • Predicted band size : 50 kDa

    Western blot detection of recombinant Human EBP50 using ab3452.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50 with ab3452.

Anti-EBP50 antibody (ab3452)参考文献

This product has been referenced in:
  • Sneddon WB  et al. Convergent Signaling Pathways Regulate Parathyroid Hormone and Fibroblast Growth Factor-23 Action on NPT2A-mediated Phosphate Transport. J Biol Chem 291:18632-42 (2016). Read more (PubMed: 27432882) »
  • Dudakovic A  et al. Histone deacetylase inhibition destabilizes the multi-potent state of uncommitted adipose-derived mesenchymal stromal cells. J Cell Physiol 230:52-62 (2015). WB ; Human . Read more (PubMed: 24912092) »

See all 21 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Sample Mouse Cell (hepatocytes)
Specification hepatocytes
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 17 2014

Application Western blot
Sample Cow Tissue lysate - whole (Kidney tissue lysate)
Loading amount 20 µg
Specification Kidney tissue lysate
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 20 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 25 µg
Specification liver
Gel Running Conditions Reduced Denaturing (8)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 30 2008

Thank you for your patience in this matter. I have received a reply from the originator of ab3452 (EBP50 antibody). The epitope recognized by this product is amino acids 286-302 of the human EBP50 protein. This information has been added to the pr...

Read More

I'm sorry to hear you are having problems with ab3452 and ab9526 and you had to re-send us your protocol details, I appreciate you taking the time to let us know those. I would like to recommend incubating the primary antibodies overnight at 4C and ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"