概述

  • 产品名称Anti-EBP1抗体
    参阅全部 EBP1 一抗
  • 描述
    兔多克隆抗体to EBP1
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human EBP1.

    (Peptide available as ab35423.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Heart (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate

性能

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应用

Our Abpromise guarantee covers the use of ab35424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

靶标

  • 功能May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
  • 组织特异性Expressed in several cell lines tested, including primary and transformed cell lines.
  • 序列相似性Belongs to the peptidase M24 family.
  • 翻译后修饰Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
  • 细胞定位Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 38kDa antibody
    • AA672939 antibody
    • Cell cycle protein p38 2G4 homolog antibody
    • Cell cycle protein p38-2G4 homolog antibody
    • ErbB-3 binding protein 1 antibody
    • ErbB3 binding protein 1 antibody
    • ErbB3-binding protein 1 antibody
    • ErbB3-binding protein Ebp1 antibody
    • hG4 1 antibody
    • hG4-1 antibody
    • IRES-specific cellular trans-acting factor 45 kDa antibody
    • MGC81621 antibody
    • MGC94070 antibody
    • Mpp1 antibody
    • p38 2G4 antibody
    • Pa2g4 antibody
    • PA2G4_HUMAN antibody
    • Plfap antibody
    • Proliferation associated 2G4 antibody
    • Proliferation associated 2G4, 38-KD antibody
    • Proliferation-associated 2G4, 38kDa antibody
    • Proliferation-associated 2G4, a antibody
    • Proliferation-associated protein 1 antibody
    • Proliferation-associated protein 2G4 antibody
    • Protein p38-2G4 antibody
    • si:dz150i12.2 antibody
    • wu:fb19b11 antibody
    • wu:ft56d05 antibody
    • zgc:86732 antibody
    see all

Anti-EBP1 antibody 图像

  • Lane 1 : Marker
    Lanes 2 - 3 : Anti-EBP1 antibody (ab35424) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : Jurkat whole cell lysate (ab7899) at 20 µg

    Secondary
    Lanes 2 - 3 : IRDye 680 conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution


    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44,54 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-EBP1 antibody (ab35424) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 55 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab35424 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab35424, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Image courtesy of Human Protein Atlas

    ab35424 staining in human kidney, showing staining of the tubuli cells (in brown). Paraffin embedded kidney tissue was incubated with ab35424 (1:600 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab35424 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

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Anti-EBP1 antibody (ab35424)参考文献

ab35424 has not yet been referenced specifically in any publications.

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