The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells.
Use 10µl for 106 cells or 100µl of whole blood.
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Belongs to the type I cytokine receptor family. Type 3 subfamily. Contains 2 fibronectin type-III domains.
Dendritic cells were activated with LPS (1µg/ml), IFN-gamma (10ng/ml) and TNF-alpha (100ng/ml) for 48 hours. A fraction of the dendritic cells were not activated to be used as negative control. 3.1 x 105 dendritic cells (activated = green line or resting cells = black line) were labelled with 10µl of ab84473.