Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (ASTAAQSGVG LARAHFEKQP PSNLRKSNFF HFVLAMYDRQ GQPVEVERTA) of Human Ebf4 (NP_001103984).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Transcriptional factor which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Belongs to the COE family.
Contains 1 IPT/TIG domain.
Information by UniProt
- COE4 antibody
- COE4_HUMAN antibody
- Early B cell factor 4 antibody
Western blot - Ebf4 antibody (ab87235)
Anti-Ebf4 antibody (ab87235) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
64 kDaObserved band size:
65 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
has not yet been referenced specifically in any publications.
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