概述

  • 产品名称Anti-EBF2抗体
    参阅全部 EBF2 一抗
  • 描述
    兔多克隆抗体to EBF2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: IAEALYSVPR NPSQLPALSS SPAHSGMMGI NSYGSQLGVS ISESTQGNNQ, corresponding to C terminal amino acids 397-446 of Human EBF2

  • 阳性对照
    • HepG2 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab56048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
  • 序列相似性Belongs to the COE family.
    Contains 1 IPT/TIG domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COE 2 antibody
    • COE2 antibody
    • COE2_HUMAN antibody
    • Collier Olf and EBF 2 antibody
    • Early B cell factor 2 antibody
    • Early B-cell factor 2 antibody
    • EBF 2 antibody
    • EBF-2 antibody
    • Ebf2 antibody
    • FLJ11500 antibody
    • Metencephalon mesencephalnon olfactory transcription factor 1 antibody
    • O/E 3 antibody
    • O/E3 antibody
    • OE 3 antibody
    • OE3 antibody
    • OLF 1/EBF like 3 antibody
    • Transcription factor COE2 antibody
    see all

Anti-EBF2 antibody 图像

  • Anti-EBF2 antibody (ab56048) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-EBF2 antibody (ab56048)参考文献

ab56048 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"