概述

  • 产品名称Anti-EBAG9抗体
    参阅全部 EBAG9 一抗
  • 描述
    山羊多克隆抗体to EBAG9
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    C-KEAQRLMKKEQN

    , corresponding to C terminal amino acids 195-206 of Human EBAG9

  • 阳性对照
    • A431 lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab52032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa). A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
    • 组织特异性Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
    • 结构域The coiled coil domain is necessary for the homodimerization.
    • 细胞定位Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BAG9 antibody
      • Cancer associated surface antigen antibody
      • Cancer associated surface antigen RCAS1 antibody
      • Cancer-associated surface antigen RCAS1 antibody
      • EB9 antibody
      • EBAG 9 antibody
      • EBAG9 antibody
      • Estrogen receptor binding fragment associated gene 9 protein antibody
      • Estrogen receptor binding site associated antigen 9 antibody
      • estrogen receptor binding site associated, antigen, 9 antibody
      • Estrogen receptor-binding fragment-associated gene 9 protein antibody
      • PDAF antibody
      • RCAS 1 antibody
      • RCAS1 antibody
      • RCAS1_HUMAN antibody
      • Receptor binding cancer antigen expressed on SiSo cells antibody
      • Receptor-binding cancer antigen expressed on SiSo cells antibody
      see all

    Anti-EBAG9 antibody 图像

    • All lanes : Anti-EBAG9 antibody (ab52032) at 0.2 µg/ml

      Lane 1 : A431 lysate
      Lane 2 : A431 lysate with Immunising peptide

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 24 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Anti-EBAG9 antibody (ab52032)参考文献

    This product has been referenced in:
    • Tskitishvili E  et al. The effect of tumor-associated protein RCAS1 gene silencing on blood pressure and urinary protein excretion in pregnant mouse: a pilot study. Am J Obstet Gynecol 203:364.e6-364.e12 (2010). WB ; Mouse . Read more (PubMed: 20579953) »

    See 1 Publication for this product

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