概述

  • 产品名称
  • 描述
    山羊多克隆抗体to EB3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Cow, Dog
  • 免疫原

    Synthetic peptide:

    SGRLSNVAPPCILR

    , corresponding to internal sequence amino acids 172-185 of Human EB3 (NP_036458.2).

  • 阳性对照
    • Human Brain (Cerebellum and Amygdala) and Mouse Brain lysates

性能

应用

Our Abpromise guarantee covers the use of ab99287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 32 kDa.

靶标

  • 功能
    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • 组织特异性
    Predominantly expressed in brain and muscle.
  • 序列相似性
    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • 结构域
    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • 细胞定位
    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Anti-EB3 antibody 图像

  • Anti-EB3 antibody (ab99287) at 0.3 µg/ml + Mouse brain lysates at 35 µg

    Predicted band size : 32 kDa
  • Anti-EB3 antibody (ab99287) at 0.1 µg/ml + Human brain (amygdala) lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Primary incubation was 1 hour.

实验方案

Anti-EB3 antibody (ab99287)参考文献

This product has been referenced in:
  • Oz S  et al. The NAP motif of activity-dependent neuroprotective protein (ADNP) regulates dendritic spines through microtubule end binding proteins. Mol Psychiatry 19:1115-24 (2014). Read more (PubMed: 25178163) »

See 1 Publication for this product

Product Wall

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A/G
Specification
N1E-115 mouse neuroblastoma cell line
Username

Mrs. Yanina Ivashko-Pachima

Verified customer

提交于 Aug 31 2017

DISCOUNT CODE: xxxx
Expiration date: xxxx

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC and include this code in the “Additional Comments” section so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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