概述

  • 产品名称Anti-EAPP抗体
    参阅全部 EAPP 一抗
  • 描述
    兔多克隆抗体to EAPP
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 32-142 of Human EAPP (Q56P03).

  • 阳性对照
    • Human adrenal gland tissue; Human cell line U251 MG.

性能

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应用

Our Abpromise guarantee covers the use of ab121154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

靶标

  • 功能May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression.
  • 组织特异性Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts.
  • 发展阶段During the cell cycle, expression disappears during mitosis.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1810011O16Rik antibody
    • BM-036 antibody
    • BM036 antibody
    • C14orf11 antibody
    • E2F-associated phosphoprotein antibody
    • EAPP antibody
    • EAPP_HUMAN antibody
    • RGD1309624 antibody
    see all

Anti-EAPP antibody 图像

  • ab121154 at 1/200 dilution staining EAPP in Paraffin-emebedded Human Adrenal gland tissue by Immunohistochemistry.
    Note: strong nuclear positivity in cortical cells.
  • ab121154 at 4 µg/ml staining EAPP in PFA/Triton X-100 fixed U251 MG cells by Immunofluorescence (green).
    Note; positivity in nucleus but not nucleoli and golgi apparatus.

Anti-EAPP antibody (ab121154)参考文献

ab121154 has not yet been referenced specifically in any publications.

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