• 产品名称Anti-EAP30抗体
    参阅全部 EAP30 一抗
  • 描述
    兔多克隆抗体to EAP30
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, derived from a region between amino acids 1-55 of Human EAP30.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab22768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • 功能Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4.
  • 序列相似性Belongs to the SNF8 family.
  • 细胞定位Cytoplasm. Endosome membrane. Nucleus. Late endosome membrane. Recruited to the endosome membrane to participate in vesicle formation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dot3 antibody
    • ELL associated protein of 30 kDa antibody
    • ELL-associated protein of 30 kDa antibody
    • ESCRT-II complex subunit VPS22 antibody
    • hVps22 antibody
    • snf8 antibody
    • SNF8 ESCRTII complex subunit homolog (S cerevisiae) antibody
    • SNF8_HUMAN antibody
    • Vacuolar sorting protein SNF8 antibody
    • Vacuolar-sorting protein SNF8 antibody
    • VPS22 antibody
    see all

Anti-EAP30 antibody 图像

  • Anti-EAP30 antibody (ab22768) at 5 µg/ml + HepG2 Cell Lysate

    HRP conjugated anti-Rabbit IgG diluted in 1/50,000 - 100,000

    Performed under reducing conditions.

    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • ab22768, at a 4.0-8.0 ug/ml dilution, staining human EAP30 in human stomach by immunohistochemistry, paraffin embedded tissue. Cells with positive label: epithelial cells of fundic gland (indicated with arrows) and surface mucous cells (indicated with arrow heads)
  • ab22768 staining EAP30 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
  • ab22768 staining EAP30 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

Anti-EAP30 antibody (ab22768)参考文献

ab22768 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. You have followed the protocol recommended by our datasheet including the dilutions and blocking agent. Furthermore Eap30 expression has been ...

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