Anti-EAN57抗体(ab121241)
Key features and details
- Rabbit polyclonal to EAN57
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EAN57抗体 -
描述
兔多克隆抗体to EAN57 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human EAN57 aa 101-233.
Database link: O43247 -
阳性对照
- IHC-P: Human testis tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121241于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use buffer at pH 6 for antigen retrieval. |
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
说明 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 339669 Human
- SwissProt: O43247 Human
- Unigene: 124502 Human
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别名
- C22orf33 antibody
- cE81G9.2 antibody
- Chromosome 22 open reading frame 33 antibody
see all
图片
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Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) of Human testis tissue sections labelling EAN57 with ab121241 (1/20). Antigen retrieval was by HIER pH6. Immunohistochemical staining shows strong cytoplasmic and nuclear positivity in a subset of cells in seminiferus ducts.
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All lanes : Anti-EAN57 antibody (ab121241) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121241 被引用在 1 文献中.
- Zhu Z et al. Spermatogenesis is normal in Tex33 knockout mice. PeerJ 8:e9629 (2020). PubMed: 32821546