重组Anti-EAF2抗体[EPR7117(2)] (ab151692)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7117(2)] to EAF2
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EAF2抗体[EPR7117(2)]
参阅全部 EAF2 一抗 -
描述
兔单克隆抗体[EPR7117(2)] to EAF2 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human EAF2 aa 150-250. The exact sequence is proprietary.
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阳性对照
- HepG2, A341 and 293T cell lysates; 293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR7117(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab151692于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. -
组织特异性
Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes. -
序列相似性
Belongs to the EAF family. -
细胞定位
Nucleus speckle. Colocalizes with ELL to nuclear speckles. - Information by UniProt
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数据库链接
- Entrez Gene: 55840 Human
- Entrez Gene: 106389 Mouse
- Entrez Gene: 266787 Rat
- Omim: 607659 Human
- SwissProt: Q96CJ1 Human
- SwissProt: Q91ZD6 Mouse
- SwissProt: Q811X5 Rat
- Unigene: 477325 Human
see all -
别名
- BM 040 antibody
- BM040 antibody
- EAF 2 antibody
see all
图片
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All lanes : Anti-EAF2 antibody [EPR7117(2)] (ab151692) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa -
Intracellular flow cytometric analysis of permeabilized 293T cells labeling EAF2 with ab151692 at 1/100 dilution (red) compared to a nonspecific control antibody (green).
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab151692 被引用在 2 文献中.
- Feng ML et al. Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer. World J Gastrointest Oncol 14:1949-1967 (2022). PubMed: 36310706
- Liu X et al. ELL Protein-associated Factor 2 (EAF2) Inhibits Transforming Growth Factor ß Signaling through a Direct Interaction with Smad3. J Biol Chem 290:25933-45 (2015). PubMed: 26370086