概述

  • 产品名称Anti-EAF2抗体
    参阅全部 EAF2 一抗
  • 描述
    兔多克隆抗体to EAF2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    AGFSHLDRRERV

    , corresponding to amino acids 5-16 of Human EAF2.

  • 阳性对照
    • Rat and mouse liver for Western blot.

性能

应用

Our Abpromise guarantee covers the use of ab28357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 2.5 µg/ml.
    WB: Use at a concentration of 1.5 µg/ml. Predicted molecular weight: 29 kDa. This protein migrates to around 43kDa when a western blot is performed. We have found that the same happened in other literature as well. A paper published by Federico Simone shows that this protein can migrate to 43kDa (see references).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
    • 组织特异性Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
    • 序列相似性Belongs to the EAF family.
    • 细胞定位Nucleus speckle. Colocalizes with ELL to nuclear speckles.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BM 040 antibody
      • BM040 antibody
      • EAF 2 antibody
      • EAF2 antibody
      • EAF2_HUMAN antibody
      • Ehrlich S II transcriptional activator factor antibody
      • ELL associated factor 2 antibody
      • ELL-associated factor 2 antibody
      • Festa antibody
      • Testosterone regulated apoptosis inducer and tumor suppressor antibody
      • Testosterone regulated apoptosis inducer and tumor suppressor protein antibody
      • Testosterone-regulated apoptosis inducer and tumor suppressor protein antibody
      • TRAITS antibody
      • U19 antibody
      • Uncharacterized bone marrow protein BM 040 antibody
      • Uncharacterized bone marrow protein BM040 antibody
      see all

    Anti-EAF2 antibody (ab28357)参考文献

    ab28357 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (293T cell lysate)
    Loading amount 30 µg
    Specification 293T cell lysate
    Treatment Transfected with EAF2
    Gel Running Conditions Non-reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Laura Pascal

    Verified customer

    提交于 May 06 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"