概述

  • 产品名称Anti-EAAT4抗体
    参阅全部 EAAT4 一抗
  • 描述
    兔多克隆抗体to EAAT4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human EAAT4.

    (Peptide available as ab45367.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Cerebellum tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab41650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 mg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 61 kDa).

靶标

  • 相关性EAAT4 is expressed predominantly in the cerebellum (Purkinje cells) and weakly in the forebrain, where it transports L-glutamate, L-aspartate and D-aspartate, acting as a symport by cotransporting sodium. EAAT4 may function as a combined transporter and inhibitory glutamate receptor and also functions as an anion-selective ion channel.
  • 细胞定位Membrane; multi-pass membrane protein.
  • 数据库链接
  • 别名
    • Excitatory amino acid transporter 4 antibody
    • High affinity neuronal glutamate transporter antibody
    • MGC33092 antibody
    • MGC43671 antibody
    • Sodium dependent glutamate/aspartate transporter antibody
    • Solute carrier family 1 (high affinity aspartate/glutamate transporter) member 6 antibody
    • Solute carrier family 1 member 6 antibody
    see all

Anti-EAAT4 antibody 图像

  • Anti-EAAT4 antibody (ab41650) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-EAAT4 antibody (ab41650)参考文献

This product has been referenced in:
  • Ramachandran PS  et al. Nonallele Specific Silencing of Ataxin-7 Improves Disease Phenotypes in a Mouse Model of SCA7. Mol Ther N/A:N/A (2014). Read more (PubMed: 24930601) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 16 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 20 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 20 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Marmoset cerebellum)
Specification Marmoset cerebellum
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"