重组Anti-EAAT3抗体[EPR6774(B)] (ab124802)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6774(B)] to EAAT3
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EAAT3抗体[EPR6774(B)]
参阅全部 EAAT3 一抗 -
描述
兔单克隆抗体[EPR6774(B)] to EAAT3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.
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阳性对照
- WB: Human fetal brain, human brain and human testis lysates . IHC-P: Human kidney tissue, and human glioma tissue. Flow Cyt: U-87 MG cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.80 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6774(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
WB | (1) |
1/10000 - 1/50000.
Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
说明 |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
靶标
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功能
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. -
组织特异性
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). -
序列相似性
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily. -
翻译后修饰
Glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6505 Human
- Omim: 133550 Human
- SwissProt: P43005 Human
- Unigene: 444915 Human
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别名
- EAA3_HUMAN antibody
- EAAC 1 antibody
- EAAC1 antibody
see all
图片
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All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution ((unpurified))
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and Diluting buffer : 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling EAAT3 with purified ab124802 at 1/1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?We are unsure how to define the extra bands.
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Unpurified ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab124802 被引用在 16 文献中.
- Meira W et al. A Cystine-Cysteine Intercellular Shuttle Prevents Ferroptosis in xCTKO Pancreatic Ductal Adenocarcinoma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33801101
- Kinoshita C et al. Inhibition of miR-96-5p in the mouse brain increases glutathione levels by altering NOVA1 expression. Commun Biol 4:182 (2021). PubMed: 33568779
- Xiong J et al. SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential. EBioMedicine 72:103614 (2021). PubMed: 34628354
- Dabrowska-Bouta B et al. Early Postnatal Exposure to a Low Dose of Nanoparticulate Silver Induces Alterations in Glutamate Transporters in Brain of Immature Rats. Int J Mol Sci 21:N/A (2020). PubMed: 33256007
- Liu XX et al. Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis. J Exp Med 217:N/A (2020). PubMed: 31699822