概述

  • 产品名称Anti-EAAT2抗体
    参阅全部 EAAT2 一抗
  • 描述
    小鼠单克隆抗体to EAAT2
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cat, Dog, Orangutan
  • 免疫原

    Recombinant fragment: DEVSSLDAFL DLIRNLFPEN LVQACFQQIQ TVTKKVLVAP PPDEEANATS AVVSLLNETV TEVPEETKMV IKKGLEFKDG, corresponding to amino acids 160 - 239 of Human EAAT2 (NP_004162) with a 26 kDa tag

  • 阳性对照
    • HeLa cell lysate; immunogen This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab77039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged EAAT2 is approximately 0.03ng/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • 序列相似性Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • 翻译后修饰Glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all

Anti-EAAT2 antibody 图像

  • IHC image of EAAT2 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77039, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-EAAT2 antibody (ab77039) at 1/500 dilution + recombinant tagged EAAT2 (the immunogen) at 0.2 µg/ml

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 62 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-EAAT2 antibody (ab77039) at 1/500 dilution + HeLa cell lysate expressing EAAT2 at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • Capture ELISA using ab77039 and recombinant tagged EAAT2

Anti-EAAT2 antibody (ab77039)参考文献

This product has been referenced in:

See 1 Publication for this product

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