概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to EAAT2
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cat, Dog, Orangutan
  • 免疫原

    Recombinant fragment: DEVSSLDAFL DLIRNLFPEN LVQACFQQIQ TVTKKVLVAP PPDEEANATS AVVSLLNETV TEVPEETKMV IKKGLEFKDG, corresponding to amino acids 160 - 239 of Human EAAT2 (NP_004162) with a 26 kDa tag

  • 阳性对照
    • HeLa cell lysate; immunogen This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab203130 as a replacement.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged EAAT2 is approximately 0.03ng/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • 序列相似性
    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • 翻译后修饰
    Glycosylated.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all

图片

  • IHC image of EAAT2 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77039, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-EAAT2 antibody (ab77039) at 1/500 dilution + recombinant tagged EAAT2 (the immunogen) at 0.2 µg/ml

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 62 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-EAAT2 antibody (ab77039) at 1/500 dilution + HeLa cell lysate expressing EAAT2 at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 62 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • Capture ELISA using ab77039 and recombinant tagged EAAT2

文献

This product has been referenced in:
  • Marignier R  et al. Neuromyelitis optica study model based on chronic infusion of autoantibodies in rat cerebrospinal fluid. J Neuroinflammation 13:111 (2016). Read more (PubMed: 27193196) »
  • Pál I  et al. Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity. Mol Brain 8:35 (2015). IHC . Read more (PubMed: 26043770) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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