Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Sample
Human Cell lysate - whole cell (endothelial cells)
Specification
endothelial cells
Treatment
vegf stimulated
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/500
Detection
Detection method
colorimetric- HRP
Bands
Specific: 140 kDa Non-specific: 250 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
VEGF treated huvecs
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Vidhya Ramaswamy
Verified customer
提交于 Sep 30 2014