Anti-eNOS抗体[6H2] (ab91205)
Key features and details
- Mouse monoclonal [6H2] to eNOS
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-eNOS抗体[6H2]
参阅全部 eNOS 一抗 -
描述
小鼠单克隆抗体[6H2] to eNOS -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Purified recombinant fragment of Human eNOS expressed in E. coli.
-
阳性对照
- WB: HuVEC Human lymph node, colon cancer, stomach cancer, and ovary cancer.
-
常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR129588-16 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
6H2 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91205于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/200 - 1/1000.
|
Flow Cyt |
1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
组织特异性
Platelets, placenta, liver and kidney. -
疾病相关
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
翻译后修饰
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
细胞定位
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
-
数据库链接
- Entrez Gene: 4846 Human
- Omim: 163729 Human
- SwissProt: P29474 Human
- Unigene: 647092 Human
-
别名
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
图片
-
Overlay histogram showing HEK293 cells stained with ab91205 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91205, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
-
ab91205, at a 1/200 dilution, staining eNOS in paraffin embedded Human lymph node (left) and colon cancer (right) tissues by Immunohistochemistry. Detection was by DAB staining.
-
ab91205, at a 1/200 dilution, staining eNOS in paraffin embedded Human stomach cancer (left) and ovary cancer (right) tissues by Immunohistochemistry. Detection was by DAB staining.
实验方案
数据表及文件
-
Datasheet download
文献 (1)
ab91205 被引用在 1 文献中.
- Osgood MJ et al. Surgical vein graft preparation promotes cellular dysfunction, oxidative stress, and intimal hyperplasia in human saphenous vein. J Vasc Surg N/A:N/A (2013). PubMed: 23911244