Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse embryonic Fibroblast)
Loading amount
25 µg
Specification
Mouse embryonic Fibroblast
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: LI-COR Goat anti-rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
LI-COR Odyssey scanner
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 47 kDa Non-specific: 60 kDa
Positive control
HeLa lysate
Additional data
Additional Notes
compared to same amount of HeLa lysate, apparantly MEF have very limited expression of eIF4A3. Nevertheless, the band migration seems similar and antibody seems specific.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 29 2011