Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (CCE))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Treatment
shRNA knockdown
Specification
mouse embryonic stem cells (CCE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% non fat milk (diluted in PBS)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 47 kDa
Positive control
Vinculin
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提交于 Jan 26 2017