Anti-eIF3K抗体[2313C2a] (ab50736)
Key features and details
- Mouse monoclonal [2313C2a] to eIF3K
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-eIF3K抗体[2313C2a]
参阅全部 eIF3K 一抗 -
描述
小鼠单克隆抗体[2313C2a] to eIF3K -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant human EIF3S12
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阳性对照
- WB: HEK293 and THP1 cell lysates; Recombinant human EIF3S12.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
克隆
单克隆 -
克隆编号
2313C2a -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50736于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
靶标
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功能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
组织特异性
Ubiquitous, with the highest levels of expression in brain, testis and kidney. -
序列相似性
Belongs to the eIF-3 subunit K family. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 27335 Human
- Omim: 609596 Human
- SwissProt: Q9UBQ5 Human
- Unigene: 314359 Human
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别名
- ARG134 antibody
- eIF 3 p25 antibody
- eIF 3 p28 antibody
see all
图片
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All lanes : Anti-eIF3K antibody [2313C2a] (ab50736) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : THP1 (Human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaLanes 1-3: Merged signal (red and green). Green - ab50736 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa.
ab50736 Anti-eIF3K antibody [2313C2a] was shown to specifically react with eIF3K in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266841 (knockout cell lysate ab258857) was used. Wild-type and eIF3K knockout samples were subjected to SDS-PAGE. ab50736 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein
Predicted band size: 25 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (1)
ab50736 被引用在 1 文献中.
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046