Anti-E3 SUMO-protein ligase PIAS4抗体(ab13795)


  • 产品名称Anti-E3 SUMO-protein ligase PIAS4抗体
    参阅全部 E3 SUMO-protein ligase PIAS4 一抗
  • 描述
    兔多克隆抗体to E3 SUMO-protein ligase PIAS4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptides QLVQFDCSPELFKKIKE and KAVQVVLRICYS, corresponding to amino acids 44-60 and 187-198 of Human PAIAS gamma.



Our Abpromise guarantee covers the use of ab13795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).


  • 功能Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
  • 组织特异性Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
  • 通路Protein modification; protein sumoylation.
  • 序列相似性Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • 结构域The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • 翻译后修饰Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • 细胞定位Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 SUMO protein ligase PIAS4 antibody
    • E3 SUMO-protein ligase PIAS4 antibody
    • FLJ12419 antibody
    • MGC35296 antibody
    • PAIASgamma antibody
    • PIAS 4 antibody
    • PIAS gamma antibody
    • PIAS-gamma antibody
    • Pias4 antibody
    • PIAS4_HUMAN antibody
    • PIASG antibody
    • PIASgamma antibody
    • PIASy antibody
    • Protein inhibitor of activated STAT 4 antibody
    • Protein inhibitor of activated STAT protein 4 antibody
    • Protein inhibitor of activated STAT protein gamma antibody
    • Protein inhibitor of activated STAT protein PIASy antibody
    • Zinc finger MIZ type containing 6 antibody
    • ZMIZ 6 antibody
    • ZMIZ6 antibody
    see all

Anti-E3 SUMO-protein ligase PIAS4 antibody 图像

  • Predicted band size : 57 kDa
  • ab13795 at 1/50 staining human fibroblasts by ICC/IF. The cells were fixed with formaldehyde and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.

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Anti-E3 SUMO-protein ligase PIAS4 antibody (ab13795)参考文献

This product has been referenced in:
  • Nath AK  et al. Proteomic-based detection of a protein cluster dysregulated during cardiovascular development identifies biomarkers of congenital heart defects. PLoS One 4:e4221 (2009). Read more (PubMed: 19156209) »

See 1 Publication for this product

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Thank you for your enquiry regarding ab13795 and for taking the time to provide some useful details of the experiments as attachments. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblasts)
Specification fibroblasts
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%

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提交于 Apr 03 2007