概述

  • 产品名称Anti-E2F6抗体[TFE61]
    参阅全部 E2F6 一抗
  • 描述
    小鼠单克隆抗体[TFE61] to E2F6
  • 经测试应用适用于: IP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human)

  • 阳性对照
    • Hela cells or K-562 nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab11952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
ICC/IF
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 38 kDa from K-562 nuclear extract(predicted molecular weight: 32 kDa). For optimal results, primary antibody incubations should be performed at 37°C

    ICC/IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
    • 组织特异性Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
    • 序列相似性Belongs to the E2F/DP family.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E2F 6 antibody
      • E2F binding site modulating activity protein antibody
      • E2F transcription factor 6 antibody
      • E2F transcription factor 6 isoform 1 antibody
      • E2F-6 antibody
      • E2F6 antibody
      • E2F6_HUMAN antibody
      • EMA antibody
      • MGC111545 antibody
      • Transcription factor E2F6 antibody
      see all

    Anti-E2F6 antibody [TFE61] 图像



    • Predicted band size : 32 kDa


      Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.

    Anti-E2F6 antibody [TFE61] (ab11952)参考文献

    This product has been referenced in:
    • Furuta M  et al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB ; Human . Read more (PubMed: 19843643) »
    • Kozaki K  et al. Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res 68:2094-105 (2008). WB ; Human . Read more (PubMed: 18381414) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"