概述

  • 产品名称Anti-E2F6抗体- ChIP Grade
    参阅全部 E2F6 一抗
  • 描述
    兔多克隆抗体to E2F6 - ChIP Grade
  • 特异性This antibody detects endogenous levels of total E2F6 protein.
  • 经测试应用适用于: IP, ChIP, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthesized peptide derived from human E2F6.

  • 阳性对照
    • Extracts from HepG2 cells.

性能

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应用

Our Abpromise guarantee covers the use of ab53061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution. PubMed: 20439742
ChIP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ELISA 1/20000.

靶标

  • 功能Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
  • 组织特异性Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
  • 序列相似性Belongs to the E2F/DP family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E2F 6 antibody
    • E2F binding site modulating activity protein antibody
    • E2F transcription factor 6 antibody
    • E2F transcription factor 6 isoform 1 antibody
    • E2F-6 antibody
    • E2F6 antibody
    • E2F6_HUMAN antibody
    • EMA antibody
    • MGC111545 antibody
    • Transcription factor E2F6 antibody
    see all

Anti-E2F6 antibody - ChIP Grade 图像

  • All lanes : Anti-E2F6 antibody - ChIP Grade (ab53061) at 1/500 dilution

    Lane 1 : Extracts of HepG2 cells
    Lane 2 : Extracts of HepG2 cells treated with peptide


    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • All lanes : Anti-E2F6 antibody - ChIP Grade (ab53061) at 1/500 dilution (in TBS fro 16 hours at 4°C )

    Lane 1 : HeLa cells
    Lane 2 : Cell lysate (nuclear) of mouse embryonic stem cells at 100000 cells

    Secondary
    HRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Dr. Ligia Tavares

  • ChIP analysis using ab53061 binding E2F6 in Mouse ES cell nuclear lysate. Samples were fixed with formaldehyde for 10 mins and incubated with primary antibody for 16 hours at 4°C at an antibody concentration of 1/500. Protein binding was detected using real-time PCR at both positive and negative control regions.

    See Abreview

Anti-E2F6 antibody - ChIP Grade (ab53061)参考文献

This product has been referenced in:
  • Roy-Chaudhuri B  et al. Regulation of microRNA-mediated gene silencing by microRNA precursors. Nat Struct Mol Biol 21:825-32 (2014). Read more (PubMed: 25086740) »
  • Velasco G  et al. Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. Proc Natl Acad Sci U S A 107:9281-6 (2010). WB, IP ; Human . Read more (PubMed: 20439742) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Human Cultured Cells (spontaneous differentiating iPSC)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification spontaneous differentiating iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Feb 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (ES cells)
Specification ES cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyd
Detection step Real-time PCR
Positive control Tuba3
Negative control Actin
Username

Abcam user community

Verified customer

提交于 Apr 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (mouse embryonic stem cells)
Loading amount 100000 cells
Specification mouse embryonic stem cells
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Dr. Ligia Tavares

Verified customer

提交于 Sep 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 40 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Dec 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"