概述

  • 产品名称Anti-E2F4抗体[WUF3]
    参阅全部 E2F4 一抗
  • 描述
    小鼠单克隆抗体[WUF3] to E2F4
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein (Human).

  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

性能

应用

Our Abpromise guarantee covers the use of ab11845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
EMSA
WB
IP
  • 应用说明GSA: Use at an assay dependent dilution.
    IP (denatured): Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µ g/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F4. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa) (for natural molecule, may vary in a recombinant preparation).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
    • 组织特异性Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • 序列相似性Belongs to the E2F/DP family.
    • 发展阶段Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
    • 翻译后修饰Differentially phosphorylated in vivo.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E2F 4 antibody
      • E2F transcription factor 4 antibody
      • E2F transcription factor 4 p107/p130 binding antibody
      • E2F-4 antibody
      • E2F4 antibody
      • E2F4_HUMAN antibody
      • p107/p130 binding protein antibody
      • Transcription factor E2F 4 antibody
      • Transcription factor E2F4 antibody
      see all

    Anti-E2F4 antibody [WUF3] (ab11845)参考文献

    ab11845 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"