Anti-E2F4抗体[WUF11] (ab11951)

概述

  • 产品名称
    Anti-E2F4抗体[WUF11]
    参阅全部 E2F4 一抗
  • 描述
    小鼠单克隆抗体[WUF11] to E2F4
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full-length protein corresponding to human E2F-4.

  • 阳性对照
    • K-562 nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab11951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Uncharacterized bands of lower and higher molecular weight may also be revealed under similar conditions. For optimal results primary antibody incubations should be performed at 37°C

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
    • 组织特异性
      Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • 序列相似性
      Belongs to the E2F/DP family.
    • 发展阶段
      Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
    • 翻译后修饰
      Differentially phosphorylated in vivo.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • E2F 4 antibody
      • E2F transcription factor 4 antibody
      • E2F transcription factor 4 p107/p130 binding antibody
      • E2F-4 antibody
      • E2F4 antibody
      • E2F4_HUMAN antibody
      • p107/p130 binding protein antibody
      • Transcription factor E2F 4 antibody
      • Transcription factor E2F4 antibody
      see all

    图片



    • Predicted band size : 45 kDa


      The presence of E2F-4 in K-562 nuclear extracts is confirmed by the detection of a signal corresponding to a protein of approximately 60 kD using ab11951 at a 1/1000 dilution.

    实验方案

    文献

    ab11951 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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