概述

  • 产品名称Anti-E2F3抗体
    参阅全部 E2F3 一抗
  • 描述
    兔多克隆抗体to E2F3
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: LLQQTEDQIP SNLEGPFVNL LPPLLQEDYL LSLGEEEGIS DLFDAYDLEK, corresponding to amino acids 406-455 of Human E2F3

  • 阳性对照
    • Fetal thymus lysate.

性能

应用

Our Abpromise guarantee covers the use of ab50917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 21297112
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

靶标

Anti-E2F3 antibody 图像

  • Anti-E2F3 antibody (ab50917) at 0.25 µg/ml + Fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab50917 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab50917 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab50917, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-E2F3 antibody (ab50917)参考文献

This product has been referenced in:
  • Giangreco AA  et al. Tumor suppressor microRNAs, miR-100 and -125b, are regulated by 1,25-dihydroxyvitamin D in primary prostate cells and in patient tissue. Cancer Prev Res (Phila) 6:483-94 (2013). WB . Read more (PubMed: 23503652) »
  • Melcher R  et al. LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability. Carcinogenesis 32:636-42 (2011). IHC-P ; Human . Read more (PubMed: 21297112) »

See all 3 Publications for this product

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