概述

  • 产品名称
    Anti-E2F1 (phospho S364)抗体
    参阅全部 E2F1 一抗
  • 描述
    兔多克隆抗体to E2F1 (phospho S364)
  • 宿主
    Rabbit
  • 特异性
    This antibody is specific for the phosphorylated form of E2F1. Minimal cross-reactivity is seen with non-phosphorylated human E2F1 by peptide ELISA.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide: SRMGSpLRAPV-C conjugated to KLH, corresponding to amino acids 360-369 of Human E2F 1.

性能

应用

Our Abpromise guarantee covers the use of ab5391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能
    Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • 序列相似性
    Belongs to the E2F/DP family.
  • 翻译后修饰
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

图片

  • All lanes : Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution

    Lane 1 : CRE (mouse) cells transfected with human E2F1
    Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin
    Lane 3 : NiH3T3 (mouse) cell lysate
    Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin
    Lane 5 : MDA-231 (human breast cancer) cell lysate
    Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin

    Secondary
    All lanes : HRP conjugated Goat-anti-Rabbit IgG MX10

    Predicted band size: 47 kDa



    Lysates were prepared from untreated cells and cells treated with 2µM Doxorubicin used as a DNA damaging agent. The arrow indicates E2F1.

     Phosphorylated E2F1 was only expressed in the treated cells. The blot clearly shows that ab5391 only detected phosphorylated E2F1 in the human cell lines.

  • ab5391 at 10 μg/ml staining human breast carcinoma.

实验方案

文献

This product has been referenced in:
  • Laine A  et al. Senescence sensitivity of breast cancer cells is defined by positive feedback loop between CIP2A and E2F1. Cancer Discov 3:182-97 (2013). Human . Read more (PubMed: 23306062) »
  • de Olano N  et al. The p38 MAPK-MK2 Axis Regulates E2F1 and FOXM1 Expression after Epirubicin Treatment. Mol Cancer Res 10:1189-202 (2012). Human . Read more (PubMed: 22802261) »

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HT29 tumour cell line)
Loading amount
50 µg
Specification
HT29 tumour cell line
Treatment
50um etoposide for 6 hr
Gel Running Conditions
Reduced Denaturing (10% tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Paul Eve

Verified customer

提交于 Apr 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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