概述

  • 产品名称Anti-E2F1 (phospho S364)抗体
    参阅全部 E2F1 一抗
  • 描述
    兔多克隆抗体to E2F1 (phospho S364)
  • 特异性This antibody is specific for the phosphorylated form of E2F1. Minimal cross-reactivity is seen with non-phosphorylated human E2F1 by peptide ELISA.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 免疫原

    Synthetic peptide: SRMGSpLRAPV-C conjugated to KLH, corresponding to amino acids 360-369 of Human E2F 1.

性能

应用

Our Abpromise guarantee covers the use of ab5391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • 序列相似性Belongs to the E2F/DP family.
  • 翻译后修饰Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

Anti-E2F1 (phospho S364) antibody 图像

  • All lanes : Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution

    Lane 1 : CRE (mouse) cells transfected with human E2F1
    Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin
    Lane 3 : NiH3T3 (mouse) cell lysate
    Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin
    Lane 5 : MDA-231 (human breast cancer) cell lysate
    Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin

    Secondary
    HRP conjugated Goat-anti-Rabbit IgG MX10

    Predicted band size : 47 kDa
  • ab5391 at 10 μg/ml staining human breast carcinoma.

Anti-E2F1 (phospho S364) antibody (ab5391)参考文献

This product has been referenced in:
  • Laine A  et al. Senescence sensitivity of breast cancer cells is defined by positive feedback loop between CIP2A and E2F1. Cancer Discov 3:182-97 (2013). Human . Read more (PubMed: 23306062) »
  • de Olano N  et al. The p38 MAPK-MK2 Axis Regulates E2F1 and FOXM1 Expression after Epirubicin Treatment. Mol Cancer Res 10:1189-202 (2012). Human . Read more (PubMed: 22802261) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT29 tumour cell line)
Loading amount 50 µg
Specification HT29 tumour cell line
Treatment 50um etoposide for 6 hr
Gel Running Conditions Reduced Denaturing (10% tris glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Paul Eve

Verified customer

提交于 Apr 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"