概述

  • 产品名称Anti-E2F1抗体[KH95] (Biotin)
    参阅全部 E2F1 一抗
  • 描述
    小鼠单克隆抗体[KH95] to E2F1 (Biotin)
  • 偶联物Biotin
  • 特异性We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant Human E2F1 protein.

  • 表位aa 342-386
  • 阳性对照
    • Mad109 or HeLa cells. Tonsil or breast carcinomas.

性能

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应用

Our Abpromise guarantee covers the use of ab79807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
IHC-P
ICC/IF
  • 应用说明ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/50 - 1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at 2µg/mg of lysate. Native and denatured.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 47 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
    • 序列相似性Belongs to the E2F/DP family.
    • 翻译后修饰Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
      Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dmel\CG6376 antibody
      • Dmel_CG6376 antibody
      • drosE2F1 antibody
      • E(Sev-CycE)3A antibody
      • E(var)3-93E antibody
      • E2-promoter binding facto antibody
      • E2F 1 antibody
      • E2F transcription factor 1 antibody
      • E2F-1 antibody
      • E2f-PA antibody
      • E2f-PB antibody
      • E2f-PC antibody
      • E2F1 antibody
      • E2f1 E2F transcription factor 1 antibody
      • E2F1_HUMAN antibody
      • Evar(3)164 antibody
      • KIAA4009 antibody
      • l(3)07172 antibody
      • l(3)j3B1 antibody
      • l(3)j3C2 antibody
      • l(3)rM729 antibody
      • mKIAA4009 antibody
      • OTTHUMP00000030661 antibody
      • PBR3 antibody
      • PRB binding protein E2F 1 antibody
      • PRB-binding protein E2F-1 antibody
      • RBAP 1 antibody
      • RBAP-1 antibody
      • RBAP1 antibody
      • RBBP-3 antibody
      • RBBP3 antibody
      • RBP 3 antibody
      • RBP3 antibody
      • Retinoblastoma-associated protein 1 antibody
      • Retinoblastoma-binding protein 3 antibody
      • Transcription factor E2F1 antibody
      see all

    Anti-E2F1 antibody [KH95] (Biotin) (ab79807)参考文献

    This product has been referenced in:
    • Helin K  et al. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Genes Dev 7:1850-61 (1993). Read more (PubMed: 8405995) »

    See 1 Publication for this product

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