概述

  • 产品名称
  • 描述
    兔多克隆抗体to E2F1
  • 特异性
    The antibody does not cross reacts with other proteins of the E2F family members.
  • 经测试应用
    适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    PCDPDLLLFATPQAPRPTPSAPRPALGRPPVKRRLD

    , corresponding to amino acids 58-93 of Human E2F1.

性能

应用

Our Abpromise guarantee covers the use of ab14768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能
    Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • 序列相似性
    Belongs to the E2F/DP family.
  • 翻译后修饰
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

图片

  • All lanes : Anti-E2F1 antibody (ab14768) at 1/500 dilution

    Lane 1 : HeLa cell extract, whole cell lysate
    Lane 2 : HeLa cell extract, nuclear lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit antibody

    Predicted band size : 47 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

ab14768 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HeLa cell extracts)
Loading amount
50 µg
Specification
HeLa cell extracts
Gel Running Conditions
Reduced Denaturing (10% SDS page tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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提交于 May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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