概述

  • 产品名称
  • 描述
    兔多克隆抗体to E tag
  • 经测试应用
    适用于: ICC, ELISA, WB, IP, ICC/IF, Flow Cytmore details
  • 免疫原

    Synthetic peptide: GAPVPYPDPLEPR conjugated to KLH.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA). A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction. Appropriate specificity controls were run.
WB 1/1000 - 1/3000.
IP Use at an assay dependent concentration. PubMed: 21784860
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 相关性
    E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.
  • 别名
    • E epitope tag antibody
    • GAPVPYPDPLEPR epitope tag antibody
    • GAPVPYPDPLEPR tag antibody

图片

  • ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Mont S  et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . Read more (PubMed: 27118599) »
  • Chavanayarn C  et al. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel) 4:554-67 (2012). WB . Read more (PubMed: 22852068) »

See all 5 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (live yeast cells)
Specification
live yeast cells
Preparation
Cell harvesting/tissue preparation method: harvest cells then wash with 1XPBS
Sample buffer: 1XPBS
Fixation
No fixation
Permeabilization
No
Gating Strategy
E-tag expression
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HUVEC)
Specification
HUVEC
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% triton-X100
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 13 2009

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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