E. coli Positive Control (大肠杆菌)全细胞裂解液- expressing 6X His tag protein (ab2431)

概述

  • 产品名称E. coli Positive Control (大肠杆菌)全细胞裂解液- expressing 6X His tag protein
  • 常规说明


    This lysate can be used as a positive control for Western blots using lysates containing 6X-His tagged proteins.

  • 经测试应用适用于: WBmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab2431 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution. This E. coli (strain BL21)lysate contains a recombinant C-terminal 6x-His tagged protein of 17 kDa. The his-tagged protein is monomeric and will be detected under reducing and non-reducing conditions. Use 5-20 µg per lane of a mini-gel at 12-15 % SDS-PAGE. After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and than stored at -20°C. The sample can be reused several times.

    E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein 图像

    • Western blot of 9 µg of E. coli BL21 lysate containing a 17kDa 6x-His tagged protein.  Mouse monoclonal to His tag (4D11 - ab5000) is used at 1:1000.

    E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431)参考文献

    This product has been referenced in:
    • Wright JC  et al. Enhanced peptide identification by electron transfer dissociation using an improved mascot percolator. Mol Cell Proteomics : (2012). Read more (PubMed: 22493177) »

    See 1 Publication for this product

    Product Wall

    E coli was grown in LB-Amp medium.

    The E. coli was cultured using a standard protocol and harvested by centrifugation. The pellet was resuspended in 50mM Tris, 10mM EDTA, pH8.0, treated with ultrasonic sound, centrifuged and the supernatant was finally filtered (0.22µm).

    Je peux vous confirmer que les cellules E. coli du produit ab2431 ont été préparé dans un tampon contenant 50mM Tris, 10mM EDTA, pH 8.0 et lysé par sonification ensuite. Le lysat était ensuite aliquoté et lyophilisé.

    Thank you for your inquiry. We use the following 4X sample buffer: 4 mL mQ H2O 1 mL 0.5M Tris-Cl, pH 6.8 0.8 mL Glycerol 1.6 mL 10% SDS (w/v) 0.2 mL Bromphenol blue + 400µl Mercaptoethanol (for reduced gels) If you reconstitu...

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    The E. coli lysate containing the his-tag protein is a whole bacterial cell pellet lysate. The amount of bands detected with a his-tag antibody depends on the quality of the his-tag antibody and is therefore antibody dependent.

    To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"