E. coli Positive Control (大肠杆菌)全细胞裂解液(ab5395)

概述

  • 产品名称E. coli Positive Control (大肠杆菌)全细胞裂解液
    参阅全部 E. coli Positive Control 细胞裂解液
  • 常规说明

    This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
    T7 HSV
    C-Myc
    VSV-g
    Glu-Glu
    V5
    E-tag
    FLAG (DDDDK)
    S-tag
    HA
    6x His

    GST

    Tag sequences: T7 = CMASMTGGQQMG
    HSV = QPELAPEDPEDC
    CMYC = EQKLISEEDLC
    VSV-g = YTDIEMNRLGK
    GluGlu = EYMPME
    V5 = GKPIPNPLLGLDST
    E-tag = GAPVPYPDPLEPR
    FLAG = DYKDDDDK
    HA = YPYDVPDYAC
    6xHIS = HHHHHH

     

    Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.

  • 经测试应用适用于: WBmore details

性能

  • 背景Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

应用

Our Abpromise guarantee covers the use of ab5395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
Use 0.5 - 2.0 µl for 10 - 12% gels.

E. coli Positive Control (Escherichia coli ) Whole Cell Lysate 图像

  • All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilution

    Lane 1 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
    Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
    Lane 4 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl
  • Anti-Myc tag antibody (ab107247) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)参考文献

This product has been referenced in:
  • Májek P  et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). Read more (PubMed: 24278455) »
  • Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . Read more (PubMed: 21515633) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.
We do not have an exact concentration for this material and do not have another lysate where the exact concentration of the myc-tagged protein is known.
I hope this information is helpful to you. Please do not hesit...

Read More

J'espère que vous allez bien!

Je vous écrit parce que j'ai finalement reçu les informations par rapportà la souche du lysat ab5395. Le laboratoire m'a confirmé que c'est la souche BL21. Je suis très d...

Read More

Thank you for your enquiry. Here is the sequence information I have for this product: N' GST - T7 - HSV - C-MYC - VSV-g - GluGlu - a tubulin - V5 - E-tag - Flag - S-tag - HA - 6xHIS C' T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC...

Read More

Thank you for your phone call and I apologize again for the shortage that you received in your vial of ab5395. I have arranged for a free of charge replacement to be sent to you; it is on order# 88591 and you should receive this Tuesday. Please let ...

Read More

Thank you for your enquiry. E. coli were lysed with SDS-PAGE sample buffer containing loading dye (ready for Western blot analysis) which gives the dark colour. Please take a look at the datasheet especially the Purification notes. Should your c...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"