Anti-E. coli O157抗体[3011] (ab20976)

概述

  • 产品名称
    Anti-E. coli O157抗体[3011]
    参阅全部 E. coli O157 一抗
  • 描述
    小鼠单克隆抗体[3011] to E. coli O157
  • 特异性
    Specific for E. coli serotype O157. The region recognized by this antibody is LPS oligosaccharide. Non-reactive with serotypes: O111, O125, O20 and O55 and K12.
  • 经测试应用
    适用于: IP, ICC/IF, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    E. coli 0157 whole cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    >90% pure.
  • 克隆
    单克隆
  • 克隆编号
    3011
  • 骨髓瘤
    unknown
  • 同种型
    IgG3
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution. Immunoprecipitates LPS of O157:H7. May be conjugated to colloidal gold.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. May be used as either a capture or detection antibody.
IHC-P Use at an assay dependent concentration. PubMed: 21858177

靶标

  • 相关性
    Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • 别名
    • Escherichia coli O157 antibody

Anti-E. coli O157 antibody [3011] (ab20976)参考文献

This product has been referenced in:
  • Li Y  et al. An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1. Theranostics 7:935-944 (2017). Read more (PubMed: 28382165) »
  • Etienne-Mesmin L  et al. Interactions with M Cells and Macrophages as Key Steps in the Pathogenesis of Enterohemorragic Escherichia coli Infections. PLoS One 6:e23594 (2011). IHC-P . Read more (PubMed: 21858177) »

See all 4 Publications for this product

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We would not be able to confirm the DH5alpha specifictiy of ab37204 and ab20976. However we can confirm ab25823 and ab48416 would work with DH5alpha species.

I hope this information is helpful to you. Plea...

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I'm happy to help. Please let me know if you have any further questions.

Thank you for your enquiry. We typically do not have IEF data for our antibodies. I have found this reference for you that might be of some help: http://onlinelibrary.wiley.com/doi/10.1002/elps.1150060305/abstract Please let me know if you need anyth...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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