Anti-E. coli O157抗体[3011] (ab20976)


  • 产品名称Anti-E. coli O157抗体[3011]
    参阅全部 E. coli O157 一抗
  • 描述
    小鼠单克隆抗体[3011] to E. coli O157
  • 特异性Specific for E. coli serotype O157. The region recognized by this antibody is LPS oligosaccharide. Non-reactive with serotypes: O111, O125, O20 and O55 and K12.
  • 经测试应用适用于: IP, ICC/IF, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    E. coli 0157 whole cells


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明>90% pure.
  • 克隆单克隆
  • 克隆编号3011
  • 骨髓瘤unknown
  • 同种型IgG3
  • 轻链类型unknown
  • 研究领域


Our Abpromise guarantee covers the use of ab20976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution. Immunoprecipitates LPS of O157:H7. May be conjugated to colloidal gold.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. May be used as either a capture or detection antibody.
IHC-P Use at an assay dependent concentration. PubMed: 21858177


  • 相关性Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • 别名
    • Escherichia coli O157 antibody

Anti-E. coli O157 antibody [3011] (ab20976)参考文献

This product has been referenced in:
  • Etienne-Mesmin L  et al. Interactions with M Cells and Macrophages as Key Steps in the Pathogenesis of Enterohemorragic Escherichia coli Infections. PLoS One 6:e23594 (2011). IHC-P . Read more (PubMed: 21858177) »
  • Park S  et al. Enzyme-linked immuno-strip biosensor to detect Escherichia coli O157:H7. Ultramicroscopy 108:1348-51 (2008). Read more (PubMed: 18562109) »

See all 3 Publications for this product

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We would not be able to confirm the DH5alpha specifictiy of ab37204 and ab20976. However we can confirm ab25823 and ab48416 would work with DH5alpha species.

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Thank you for your enquiry. We typically do not have IEF data for our antibodies. I have found this reference for you that might be of some help: Please let me know if you need anyth...

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