The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
See attached protocol.
E. coli is a group of gram-negative bacilli belonging to the family Enterobacteriaceae and is considered as one of the bacteria in the human intesinal normal microbial flora. Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigen (H type: H1-H56).