Anti-E. coli抗体(FITC) (ab30522)

概述

  • 产品名称Anti-E. coli抗体(FITC)
    参阅全部 E. coli 一抗
  • 描述
    兔多克隆抗体to E. coli (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Tissue/ cell preparation (Escherichia coli, all serotypes). A mixed culture of E. coli strains (O157:H7, O20, O125, O55, O111 & K12), intact plus lysed, non-denatured, was gamma-inactivated and used directly as the immunogen.

性能

应用

Our Abpromise guarantee covers the use of ab30522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/25. Acetone fixation of the antigen is recommended.

靶标

  • 相关性Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • 别名
    • Escherichia coli antibody

Anti-E. coli antibody (FITC) (ab30522)参考文献

This product has been referenced in:
  • Golberg A  et al. Cloud-enabled microscopy and droplet microfluidic platform for specific detection of Escherichia coli in water. PLoS One 9:e86341 (2014). Read more (PubMed: 24475107) »
  • Heinze BC & Yoon JY Nanoparticle immunoagglutination Rayleigh scatter assay to complement microparticle immunoagglutination Mie scatter assay in a microfluidic device. Colloids Surf B Biointerfaces 85:168-73 (2011). Read more (PubMed: 21411297) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Sample Escherichia coli Cell (Planktonic)
Permeabilization Yes - acetone
Specification Planktonic
Fixation Acetone
Username

Abcam user community

Verified customer

提交于 Aug 17 2015

Thank you for your enquiry.

It might be possible for a FITC-conjugated IgG to still be able to bind to Protein A as well, primarily due to the fact that FITC conjugation to IgG and Protein A binding to IgG occur at different sites within the...

Read More

Thank you for your patience. I can now confirm that ab30522 also cross-reacts with the DH5a strain of E.coli. I hope this information is helpful for your customer.

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Escherichia coli Cell (pure culture)
Specification pure culture
Username

Abcam user community

Verified customer

提交于 May 09 2007

Thank you for your patience. Regarding ab30522 (E.coli antibody (FITC)), a mixed culture of E. coli strains was gamma-inactivated and used directly as the immunogen. Although the concentration has not been determined, there is a dilution recommend...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"