Anti-E. coli抗体(Biotin) (ab68451)

概述

  • 产品名称
    Anti-E. coli抗体(Biotin)
    参阅全部 E. coli 一抗
  • 描述
    兔多克隆抗体to E. coli (Biotin)
  • 偶联物
    Biotin
  • 特异性
    ab68451 reacts with E. coli in bacterial and infected tissue samples.
  • 经测试应用
    适用于: ELISAmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    O and K antigenic serotypes (All types) of Escherichia coli.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • 别名
    • Escherichia coli antibody

文献

This product has been referenced in:
  • Dua P  et al. Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection Formats. Mol Cells 39:807-813 (2016). Functional Studies . Read more (PubMed: 27871171) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Escherichia coli Cell (Escherichia Coli trapping using magnetic beads der)
Permeabilization
No
Specification
Escherichia Coli trapping using magnetic beads der
Preparation
Cell harvesting/tissue preparation method: magentic streptavidin-coated beads labeled with ab68451 (biotin) to trap E. Coli from dilutions after LB culture
Sample buffer: Phosphate Buffer Saline, pH=7.4 [0.01 M with 27 mM KCl, 137 mM NaCl]
Fixation
on magnetic streptavidin beads
Username

Cedric Hurth

Verified customer

提交于 Apr 07 2017

Thank you for contacting us.

I have attached the list of antibodies we have along with details. Please check.

I am sorry I am unable to confirm the species cross reactivity of the these antibodies other than waht is given on the datas...

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Thank you for contacting us.

ab41181 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab41203 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab19980 is an anti Ent...

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Thank you so much for your patience while I have worked on this enquiry.

I've looked through the literature, data on our E.coli antibodies, and also discussed with my colleagues, and I think that the unconjugated version of ab20640 will be th...

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Thank you for your reply.

I will get in touch with the lab about the unconjugated E. coli antibodies that we have, and see if there is any data to suggest that one will work in your assay! I'll get back in touch with you as soon as possible. ...

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Thank you very much for your reply.

I apologize for the confusion regarding this situation. For the offer that I've mentioned, the "review" is our online Abreview system. So, if you'd like to submit an Abreview for ab68451 (we appre...

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Thank you very much for your reply and for sending the description of the assay in which you will be using the E. coli antibody.

We have not tested any of our E. coli antibodies in a similar assay, so there isn't one in particular that we kno...

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Thank you for your reply.

I am very sorry that we do not have free samples to send, but our testing program is the option that weprovide for trying an antibody in an untested application.

We generally arrange this program prior to p...

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Thank you very much for your reply.

At this time, we do not have the unconjugated format of ab68451 in our catalog, but if you are interested in trying it Iwill see if we can add this antibodyto ourcatalog. We also have two unconjugated goat ...

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Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.
I have confirmed with the lab that the biotinyalted antibody is tested, and I've obtained the following ELISA protocol from the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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